The Study of pH Influence on Bovine Liver Catalase by Means of UV-VIS Spectroscopy and Spin Labelling Method
نویسندگان
چکیده
The EPR and UV-VIS spectroscopy was used to determine the pH effect on bovine liver catalase. The measurements were made in the pH range from 2 to 10. The positions, shapes and relative intensities of the UVVIS peaks were observed. Spin labelling technique was used to monitor the molecular dynamics of catalase. Treatment with acid or alkaline solutions causes spectral changes which may be due to dissociation of the enzyme into subunits and removal of the haeme group from the protein. The decrease of respective absorption bands and their shifts under acid and alkaline pH correlate well with the changes of rotational correlation time
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